Prostate Cell Specific Regulation of Androgen Receptor Phosphorylation In Vivo

Abstract

On the basis of previous studies in which we analyze AR phosphorylation in vivo we propose that AR phosphorylation at serines 213 and 650 regulate differential target gene expression and recruitment to gene promoters via altered interaction with other cellular transcription factors. To test this hypothesis we have conducted yeast two-hybrid analysis with the N-terminus of wild type AR as well as AR S213A and AR S213E variants. Our preliminary analysis indicates that the screen is preferentially isolating proteins with a known role in gene transcription and we are currently assessing the phosphorylation- dependence of the putative AR interacting proteins.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2006
Accession Number
ADA465845

Entities

People

  • Brian D Dynlacht
  • Derick Mitchell
  • Michael Garabedian
  • Samir Taneja
  • Susan Logan

Organizations

  • Grossman School of Medicine

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Androgen Receptors
  • Androgens
  • Antibodies
  • Biomedical Research
  • Cell Line
  • Cells
  • Gene Expression
  • Genetic Structures
  • Macromolecules
  • Molecules
  • Neoplasms
  • New York
  • Phosphorylation
  • Prostate
  • Prostate Cancer
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics