Expression and Genomic Profiling of Minute Breast Cancer Samples
Abstract
To be able to apply the newest genetic analysis technologies in breast cancer research, enough DNA material must be available to perform this analysis. Often this is impossible with the minute amounts of tissue obtained via fine needle aspiration or laser capture microdissection currently the best methods available for removal of small amounts of tissue. Therefore whole genome-based DNA amplification techniques (e.g. PCR) are essential. The aim of this proposal was to evaluate a newly developed method, balanced PCR, which overcomes the difficulty of non-linear PCR-amplification of complex genomes and faithfully retains the difference among corresponding genes or gene fragments. In the three years of work we demonstrated the application of balanced PCR in performing genomic profiling of breast CA cell lines and samples (part of Tasks 2 and 3). In the second year we demonstrated the application of the method for gene expression profiling of breast CA cell lines (Task 1). This report describes the work conducted over the three years.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2006
- Accession Number
- ADA466110
Entities
People
- G. M. Makrigiorgos
Organizations
- Dana–Farber Cancer Institute