Identifying Molecular Factors in Androgen Receptor Nuclear Export
Abstract
Androgen receptor (AR) plays a central role in prostate cancer and the regulation of its nuclearicytoplasmic transport represents another level control of its activity. However how AR is transported into and out of the nucleus remains largely a mystery. Yeast (S. cerevisiae) provides a powerful system to study fundamental cellular mechanisms. Thus we use yeast as a model system to identify factors that are involved in the AR nuclear export. A mutagenesis screen was performed and thus far 6000 individual clones (about I yeast genome) have been examined. Seven mutants that displayed a defect in nuclear export were isolated. Preliminary results suggest that one mutant yeast clone 27.7D is a recessive gene that may play a role in the nuclear localization of AR. In addition the mutant clone 27.7D also exhibit retarded growth. The export and growth defects observed in clone 27.7D appear to be caused by the same mutation or two separnte but very closely linked mutations. Complementation experiments show that 27.7D is distinct from two other mutants clones I .3E and 72.5E which are actively being characterized. Initial results also suggest that the combination of the mutation in 27.7D and the mutation of another mutant clone I 02.5D results in lethality. This suggests the possibility that these two genes may belong to two parallel pathways.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2007
- Accession Number
- ADA466147
Entities
People
- Yujuan Weng
Organizations
- Northwestern University