Immunotherapy Targeting Tumor Stromal Fibroblasts Improves Chemotherapy of Breast Cancer

Abstract

We demonstrated that a DNA-based vaccine encoding fibroblast activation protein (FAP), overexpressed on tumor-associated fibroblasts (TAFs), induces a CD8+ T cell response killing TAFs. This effectively suppressed or eradicated primary tumors or their pulmonary metastases in a murine breast carcinoma model. Moreover, eradication of TAFs decreased the interstitial fluid pressure of the tumor since it markedly diminished collagen type I produced by TAFs. This resulted in increased tumor uptake (70%) of doxorubicin which made the combination of the pFAP vaccine with chemotherapy highly effective as it completely eradicated primary breast tumor growth in a mouse tumor model.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2007
Accession Number
ADA466673

Entities

People

  • Ralph A. Reisfeld

Organizations

  • Scripps Research

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemotherapy
  • Collagen
  • Combination Therapy
  • Liquid Chromatography
  • Lymphocytes
  • Mass Spectrometry
  • Neoplasms
  • Oncology
  • Therapy
  • Tissues
  • Vaccines
  • Wound Healing

Fields of Study

  • Biology

Readers

  • Geochemistry
  • Immunology
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech