Identification of a Protein for Prostate-Specific Infection
Abstract
In this proposal, we will identify and clone a protein that can be used to generate infection-specific gene therapy vector. We expect that using this protein to modify various gene therapy vectors, we can specifically deliver cytotoxic genes into prostate cancer cells using systemic treatment, and eventually eradicate metastatic prostate cancer cells in patients. During the third year, we inserted the sequences that encode these two peptides to modify lentiviral vector envelop gene and used one of the modified membrane proteins to target prostate cells. The viral vector that have peptide YDSVLALSAALQATR (P2) modified gp41 envelope protein was used to infect LNCaP cells in 24-well plates. The control vector that does not have gp41-P2 envelope protein on viral surface was also used to infect LNCaP cells as the control. Our results demonstrated that with the gp41-P2 envelope protein on the surface of the viral vector, lentiviral vectors increase infectivity by 30% to 70%.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2006
- Accession Number
- ADA466677
Entities
People
- Shen Pang
Organizations
- University of California, Los Angeles