Disruption of the Circadian Rhythms of Gene Expression and the Development of Breast Cancer
Abstract
This project uses a mouse model to examine the effects of shiftwork on the expression of genes that are directly involved in the genesis and progression of breast cancer. Task 1: Shift work simulation experiments in mice have been accomplished. High variability has made it advisable to increase the number of animals from n=5 per group to n=10. Results show that shift work produces a shift in the rhythm of core clock genes and the attenuation of expression of genes involved in cell cycle. A manuscript with these results is currently in preparation. Task 2: Two preliminary experiments have been carried out to assess the grafting success of MCF-7 and MDA-MB-231 tumour cells. We expect to run the full experiment within 8 weeks. Task 4: We have successfully crossed the Clock mutation on to a BALB/c-Foxn1nu background. The pups from this cross were not viable due to the inability of the mothers to lactate their offspring. This, in itself, is a very interesting finding. We have been able to maintain this colony by cross fostering the mutant pups to a lactating heterozygote mother. As a backup we have started to cross the Clock mutation on to a Severe combined immuno-defficient (SCID) mouse background with the expectation that these will not manifest the same problems.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2007
- Accession Number
- ADA467567
Entities
People
- David J. Kennaway
Organizations
- University of Adelaide