Role of Reactive Stroma in Prostate Cancer Progression
Abstract
The purpose of this project is to determine the role of FGF receptor 1 in reactive stroma during prostate tumorigenesis. We are using a novel approach to target transgene expression specifically to the reactive stroma of experimental prostate cancer. Using a modified approach, we are placing an inducible Cre recombinase behind the FAP gene promoter to target expression to reactive stroma. We will cross this mouse with Fgfr1flox mice (LoxP sites flanking FGF receptor 1 alleles). These mice will be crossed with TRAMP mice (prostate cancermodel). Induced expression of Cre at sites of reactive stroma generated in the cancer foci will function to excise the FGF receptor 1 alleles and create a conditional knockout mouse. Progression of tumorigenesis in this line of knockout mice will be compared to heterozygous and wild type controls. Progress has been made in each Task. We have completed all cloning steps and acquired all reagents. We have rederived the Fgfr1flox and have crossed it into the appropriate backgrounds. We have completed crossing the TRAMP mice with the Fgfr1flox mice. This study will pinpoint the role of FGF receptor 1 in reactive stroma promotion of prostate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2007
- Accession Number
- ADA467602
Entities
People
- David R. Rowley
Organizations
- Baylor College of Medicine