Process Research and Development of Antibodies as Countermeasures for C. Botulinum

Abstract

This report describes the project to fill out shell space on the third floor of Othmer Hall, the new home for the University of Nebraska-Lincoln Biological Process Development Facility. Construction is 95% completed and all research laboratories of the BPDF are functional. The 5% includes a Master Cell Banking. The third floor is home to all process research capabilities, i.e. molecular biology, fermentation, cell culture, analytical methods, quality control, purification development, media prep, and two academic laboratories in the department of chemical engineering performing bioengineering research. The contract for detailed design of the cGMP basement was awarded June 2004 and is expected to be completed by Fall 2005. This report also describes research on the expression of an antibody against serotype A botulinum neurotoxin in Chinese Hamster Ovary (CHO). Research on new expression plasmids, development of a serum-free media, and optimization of a fed-batch process to produce the antibody against.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2005
Accession Number
ADA467874

Entities

People

  • Michael Meagher

Organizations

  • University of Nebraska–Lincoln

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Antibodies
  • Bioengineering
  • Biological Processes
  • Biology
  • Chemical Engineering
  • Culture Media
  • Culture Techniques
  • Engineering
  • Molecular Biology
  • Quality Control
  • Research Facilities

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Nuclear Civil Defense.
  • Systems Analysis and Design

Technology Areas

  • Biotechnology
  • Space