Microarray Analysis of Transposon Insertion Mutants in Bacillus Anthracis: Global Identification of Genes Required for Sporulation and Germination

Abstract

A transposon site hybridization (TraSH) assay was developed for functional analysis of the B. anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of the pathogen's genes. The system, used to identify genes required for generation of the infectious anthrax spore, spore germination and optimal growth on rich medium, was predictive of the contribution of two conserved hypothetical genes for the phenotypes examined.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2007
Accession Number
ADA467921

Entities

People

  • Arthur M. Friedlander
  • Beth M. Carpenter
  • Scott N. Peterson
  • Suzanne L. Rasmussen
  • William A. Day Jr.

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacteria
  • Bacteriology
  • Biomedical Research
  • Demographic Cohorts
  • Diseases And Disorders
  • Functional Analysis
  • Genes
  • Genetics
  • Genome
  • Germination
  • Hybridization
  • Infectious Diseases
  • Microarray Analysis
  • Microbiology
  • Phenotypes
  • Spores
  • United States

Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Microbial Pathology