Methylselenium and Prostate Cancer Apoptosis

Abstract

The purpose of this research is to gain a better understanding of the biochemical pathways and molecular targets for the selective induction of apoptosis signaling and execution of prostate cancer (PCa) cells by methyl selenium. We hypothesized that methyl selenium inhibits PI3K-AKT survival pathway leading to the activation of caspase-dependent apoptosis execution in PCa cells. Data generated support PI3K-AKT pathway activity as a determinant of the apoptosis sensitivity of PCa cells to a prototype methyl Se MSeA which can be further modulated by ERK1/2. AKT and ERK1/2 differentially modulated cytochrome c involvement in MSeA-induced extrinsic (major) and intrinsic caspase cascades. We have pursued a novel lead for using selenium as a chemosensitizer for cancer therapeutic drug-induced apoptosis in androgen independent PCa cells. We have also discovered a synergistic enhancement of apoptosis effect of TRAIL (an anti-cancer biological) by the genotoxic selenite through p53-mediated actions. The chemosensitizer activity of selenium is expected to improve quality of life of prostate cancer patients because of increased efficacy and reduced dosage needed. Seven publications are credited to the support by this grant award. Validation of these findings in animal models is currently in progress.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2007
Accession Number
ADA467941

Entities

People

  • Junxuan Lu

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Androgens
  • Apoptosis
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Department Of Defense
  • Enzyme Inhibitors
  • Membrane Potentials
  • Models
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Proteins
  • Selenium
  • Selenium Compounds
  • Therapy

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Prostate Cancer Biology.