Protection with Recombinant Clostridium botulinum C1 and D Binding Domain Subunit (Hc) Vaccines Against C and D Neurotoxins
Abstract
Recombinant botulinum Hc (rBoNT Hc) vaccines for serotypes C1 and D were produced in the yeast Pichia pastoris and used to determine protection against four distinct BoNT C and D toxin subtypes. Mice were vaccinated with rBoNT/C1 Hc, rBoNT/D Hc, or with a combination of both vaccines and challenged with BoNT C1, D, C/D, or D/C toxin. Mice receiving monovalent vaccinations were partially or completely protected against homologous toxin and not protected against heterologous toxin. Bivalent vaccine candidates completely survived challenges from all toxins except D/C toxin. These results indicate the recombinant C1 and D Hc vaccines are not only effective in a monovalent formula but offer protection against both parental and mosaic toxins when delivered as a bivalent vaccine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 16, 2007
- Accession Number
- ADA468185
Entities
People
- Leonard A. Smith
- Michael M. Meagher
- Patrick M. Wright
- Robert P. Webb
- Theresa J. Smith
- Vicki A. Montgomery
Organizations
- United States Army Medical Research Institute of Infectious Diseases