p63 in Development and Maintenance of the Prostate Epithelium
Abstract
The purpose of this project is to define the role of p63 in the development and maintenance of the prostate epithelium by utilizing both in vivo and in vitro models. In our first year of work, we have the successful constructed the targeting vector for the generation of the p63-Cre-ERT2 knock-in mice to be used for genetic lineage tracing. The p63-Cre-ERT2 vector has been transferred to the DFCI Gene Targeting Core Facility and electroporation in the ES cells will be performed shortly. The strategy for screening of ES cells for homologous recombination has been already designed and probes for Southern Blot analysis have been generated and successfully tested. In addition, we have made progress in exploring the molecular mechanisms through which p63 regulates development of the prostate epithelium. Specifically, the use of siRNA against p63 has been optimized in various cell lines and, most importantly, p63 shRNA inducible cell lines (including iPrEC) have been generated. Preliminary results obtained utilizing these reagents show that down regulation of p63 in iPrEC cells consistently causes a decrease in cell viability.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2007
- Accession Number
- ADA468492
Entities
People
- Sabina Signoretti
Organizations
- Brigham and Women's Hospital