Cadmium, Zinc, and Selenium Levels in Carcinoma of the Human Prostate
Abstract
The purpose of this study is to examine associations between zinc (Zn), selenium (Se) and cadmium (Cd), on development of prostate cancer. The objectives of this project are: 1) to establish the reliability of using formalin-fixed paraffin embedded (FFPE) prostate tissue by comparing levels of Zn, Se and Cd between FFPE and fresh-frozen unfixed prostate tissues; 2) to enhance our knowledge of prostate cancer environmental etiology by examining the association of Cd, Zn, Se with the development and progression of cancer using FFPE tissues; 3) to enhance the use of the AFIP National Tissue Repository of archival tissues. First objective has been successfully accomplished based on the development of a method which uses high-resolution inductively-coupled mass-spectrometry (ICP-MS) for the analysis of Zn, Cd, Se including arsenic (As) and iron (Fe) in FFPE prostate tissue. Our results have demonstrated that in comparison with fresh tissue, Fe, Se and As do not show much difference in the FFPE tissue. On the other hand, Zn and Cd resulted in ~40% and ~60% loss, respectively, requiring using the correction factors to assess their content in the original tissue. The technique can be useful in various studies for analysis of archival FFPE tissue, and provides a basis for fulfilling the next aim of the project, the
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2007
- Accession Number
- ADA468497
Entities
People
- Andrey Sarafanov
- Jose A. Centeno
Organizations
- Armed Forces Institute of Pathology