Immunology, Systems Biology, and Immunotherapy of Breast Cancer
Abstract
This first year of this award has been one of intense infrastructure building. This included recruitment and training of two excellent research associates, two PhD postdoctoral fellows, and one part-time student. I spent several months working closely with Dr. Donna Ferrandino on a human subjects protocol which was approved in August by both the Stanford IRB (protocol ID 4408) and DoD HSRRB (A-13776.2). This enabled us to work closely with our surgery and pathology colleagues to develop an efficient system of identifying, recruiting, and consenting subjects, and to obtain samples from the operating room to pathology and eventually to my laboratory. We tested multiple protocols to maximize recovery of immune cells from tumor and lymph node specimens. We also optimized methods for analysis of fresh and archive samples by flow cytometry, immunohistology, immunoflourescence, function assays, and DNA microarray analysis. The attached annual report is a summary of our progress in relation to my proposed SOW.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2007
- Accession Number
- ADA468517
Entities
People
- Peter P Lee
Organizations
- Stanford University