Molecular Mechanisms in Compromised Endothelial Barrier during Breast Cancer Metastasis
Abstract
In this funding period (first of three years) we have fulfilled the scheduled tasks of (1) standardizing the condition for engineering the 3D vasculature network that can consistently produce lumen; (2) generating various viral constructs that can deliver the FRET sensors to 100% of the endothelial cells used in vasculature formation; (3) determining the fluid exchange rate within the 3D collagen matrix that will be critical for any subsequent drug treatment of the endothelial cells in the assay system; (4) co-developing with Improvision a FRET module that is capable of performing 3D ratio-imaging. We presented here to the best of our knowledge the first ever three dimensional FRET microscopy data. We have also established that the endothelial cells undergo polarization similar to those under physiological condition during in vitro vasculogenesis. Taken together these developments meet and exceed the scheduled tasks outlined in the statement of work and complete the establishment of the 3D FRET assay system needed to study the transient modulation of endothelial signaling events by breast cancer cells during transendothelial migration.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2007
- Accession Number
- ADA468692
Entities
People
- Teng-leong Chew
Organizations
- Northwestern University