Cross-Serotype Neutralization of Dengue Virus in Aotus nancymae Monkeys
Abstract
Previously, we observed that serum from humans immune to dengue serotype 1 (dengue-1) neutralized the American genotype of dengue serotype 2 (American-2) to a greater extent than it neutralized the Asian genotype of dengue serotype 2 (Asian-2). To determine if this activity is protective, Aotus nancymae monkeys were infected with dengue-1 followed by either American-2 or Asian-2. Dengue-1 infected animals produced antibody with neutralizing titers of 2656 antibodies against dengue-1, 409 against American-2, and !20 against Asian-2. Infection with American-2 did not produce detectable viremia in either dengue-1 immune or dengue- 1 naive animals. These findings support the hypothesis that dengue-1 immunity might have prevented disease or altered the severity of disease in individuals sequentially infected with dengue-1 and American-2. Dengue viruses are single-stranded, positive-sense RNA viruses that belong to the Flavivirus genus in the Flaviviridae family. There are 4 serotypes of dengue virus (dengue 1 4), and each serotype consists of many closely related (genotypic) viruses. Dengue viruses are transmitted primarily by Aedes aegypti mosquitoes, and all 4 serotypes cause human disease. It is estimated that 100 million dengue infections occur annually worldwide [1], and these can either be subclinical or cause diseases ranging from a flulike syndrome dengue fever to severe disease characterized by capillary leakage and thrombocytopenia dengue hemorrhagic fever (DHF) to hypovolemic shock dengue shock syndrome.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 2005
- Accession Number
- ADA468920
Entities
People
- Alfonso S. Gozalo
- Daniel F. Ewing
- Douglas M. Watts
- Kevin L. Russell
- Kevin R. Porter
- Tadeusz J. Kochel
Organizations
- Naval Medical Research Center