Identification, Cloning, and Expression of Potential Diagnostic Markers for Q Fever

Abstract

The clinical diagnosis of Q fever is difficult. Whole cell antigens are currently used in several serological methods, but antigens are limited due to the hazardous nature of Coxiella burnetii cultivation. In this report, we described the method of detecting immunodominant antigens of C. burnetii by using proteomic techniques with patient sera, and cloning and expressing the selected antigens using a novel vector known for its ease of expression, purification, and downstream application.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2005
Accession Number
ADA468963

Entities

People

  • B. Hanson
  • C. C. Chao
  • H. W. Chen
  • W. B. Xu
  • W. M. Ching
  • Xiangchong Li

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Diseases And Disorders
  • Electrophoresis
  • Escherichia Coli
  • Gel Electrophoresis
  • Instructions
  • Liquid Chromatography
  • Maryland
  • Mass Spectrometers
  • Mass Spectrometry
  • Navy
  • New York
  • Proteins
  • Q Fever
  • Two Dimensional

Fields of Study

  • Medicine

Readers

  • Breast cancer cell signaling and growth regulation.
  • Immunology
  • Systems Analysis and Design

Technology Areas

  • Biotechnology