A Rat Model for Human Ductal Carcinoma In Situ
Abstract
Our objective was to develop a rodent model for human OCIS and LCIS in which lesions of diverse phenotypes could be induced and characterized and their malignant potential studied in a linear manner. We tested the effect of the infusion of EGF FGF orIGF1 on the proliferation of mammary glands in ovariectomized rats. Infusion of EGF, FGF or IGF1 resulted in end bud formation and lobuloalveolar development within 72 hours. Intraductal proliferation resulted only in rats treated with IGF1. These results indicate that infusion of growth factors into the mammary glands induces proliferation of different kinds of structurns including end buds lobuloalveolar structures and intraductal proliferations and that the growth factors in ovariectomized rats can support neoplastic mammary transformation. EGF or IGF-1 were infused in ovariectomized rats using Alzet pumps and treated with the chemical carcinogen N-methyl-N nitrosourea (MNU). The cancers were varied in their estrogen and progesterone receptor expression as determined by immunocytochemistry. The objectives of the project were not achieved due to the low incidence of mammary cancers induced following treatment with growth factors and MNU making it difficult to transplant lesions of different phenotypes and characterize the malignant potential.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2006
- Accession Number
- ADA469002
Entities
People
- Satyabrata Nandi
Organizations
- University of California, Berkeley