Proteomic Analysis of Rickettsia prowazekii

Abstract

Rickettsia prowazekii is an obligate intracellular gram-negative bacterium. Comparative proteomics study of a virulent strain (Breinl) versus an avirulent strain (Madrid E) was performed using an integrated liquid chromatography and mass spectrometer. About 30% of predicted proteins were detected and identified. Among the detected proteins, more than 30 proteins were of unknown function in both strains. Although several proteins were detected in only one strain, the overall distribution of detected proteins in different COGs (clusters of orthologs groups) was very similar between the two strains. Functional analysis of differentially expressed proteins, either qualitatively or quantitatively, may lead to the discovery of pathogenesis-related factors.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2005
Accession Number
ADA469065

Entities

People

  • C. C. Chao
  • D. Chelius
  • E. Mutumanje
  • Tianqing Zhang
  • W. M. Ching

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Biological Weapons
  • Biomedical Research
  • Civil Disturbances
  • Computing-Related Activities
  • Data Analysis
  • Data Science
  • Diseases And Disorders
  • Maryland
  • Mass Spectrometers
  • Military Personnel
  • Navy
  • New York
  • Preventive Medicine
  • Proteins
  • United States
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Neurodegenerative Parkinson's Disease and Rickettsial Disease handbook, including the data level of dopamine, BC, neurons, and PD.

Technology Areas

  • Biotechnology