Characterizing Candidate Oncogenes at 8q21 in Breast Cancer
Abstract
DNA amplification is a cardinal feature of cancer and plays an important role in tumor progression by altering the gene expression program. These amplified regions are associated with oncogenes of known and unknown identity. By utilizing array comparative genomic hybridization technology to map DNA copy number changes at high resolution we have identified a recurrent region of high-level amplification at the 8q21 locus that is clinically significant in breast cancer by our preliminary analysis and has yet to be associated with a known oncogene. We have narrowed down this region to two annotated genes with unknown function. In this study we have: (1) created stable cell lines expressing shRNA constructs that will express an siRNA causing inhibition of each candidate oncogene respectively. (2) begun to generate overexpression vectors for each candidate oncogene. (3) raised antibodies to each of the candidate oncogene to further characterize the protein of each candidate oncogenes. Ultimately this novel oncogene may serve as a new drug target.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2007
- Accession Number
- ADA469206
Entities
People
- Jessica Kao
Organizations
- Stanford University