Laminin-5 gamma2 Chain in Breast Cancer Metastasis

Abstract

To metastasize, cancer cells have to break through basement membrane (BM). Laminin-5 is one of the most abundant BM proteins. It consists of three chains alpha3, beta3 and gamma2. DIII domain, a functional EGFR ligand will be released from Ln-5 2 chain by MMPs processing. It has been suggested by our lab that DIII domain may facilitate cancer progression by preventing anoikis. There are paradoxical data in regard of the role of Ln-5 in cancer progression. For example, both the increased and decreased expression levels of Ln-5 subchains are reported in the literature. The fact that gamma2 chain exists in two different forms (as a secreted monomer, or as a part of the Ln-5 heterotrimer) leads us to hypothesize that those two forms may play different roles in cancer progression. In this proposal we will determine if the expression of Ln-5gamma2 monomer is positively correlated with breast cancer cell line tumorigenecity. In addition, we will determine the role of Ln-5gamma2 chain in cancer progression when it is in the context of Ln-5 heterotrimer.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2007
Accession Number
ADA469249

Entities

People

  • Shanshan Liu

Organizations

  • Vanderbilt University Medical Center

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Azo Compounds
  • Basements
  • Biological Sciences
  • Biological Staining And Labeling
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Movement
  • Cells
  • Culture Techniques
  • Intercellular Junctions
  • Literature
  • Membranes
  • Neoplasms
  • Tissues

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Molecular Biology and Genetics
  • Oncology (Cancer Research).