Structure-Based Design of Inhibitors to the Cytotoxin Ricin

Abstract

Ricin is a cytotoxin and a known bioterrorist weapon. The Army is pursuing anti-ricin vaccines but plans to develop an efficacious antidote to the toxin for cases where vaccination is not appropriate. The goal of this project is use the X-ray structure of ricin A chain (RTA) as a template for inhibitor design. Computer modeling and X-ray screening aid in the design process. Inhibitors which bind to the RTA substrate specificity site have been identified. A platfdrm 9-deazaguanine has been shown to bind in the RTA active site and act as a weak inhibitor. However efforts to derivatize and diversify the platform via triazole %click" chemistry have met with unanticipated difficulties. None of the new compounds exhibits greatly improved inhibitory properties. A virtual screen of available compounds suggests catechols may provide a novel platform for future work.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2007
Accession Number
ADA469261

Entities

People

  • Jon Robertus

Organizations

  • University of Texas at Austin

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Algorithms
  • Analogs
  • Antidotes
  • Biomedical Research
  • Chemical Synthesis
  • Chemistry
  • Computers
  • Cytotoxins
  • Databases
  • Inhibitors
  • Molecules
  • Platforms
  • Small Molecules
  • Solubility
  • Substrates
  • Three Dimensional
  • X Rays

Fields of Study

  • Chemistry

Readers

  • Immunology
  • Molecular Genetics
  • Systems Analysis and Design

Technology Areas

  • Biotechnology