Rho GTPase Involvement in Breast Cancer Migration and Invasion

Abstract

Using a high throughput small interfering RNA approach (siRNA) I have screened 1081 human genes (kinases phosphatases and a library of migration-related genes) using an automated wound healing assay to identify genes that regulate cell migration using the normal mammary epithelial cell line MCF10A. Genes were classified into hit bins based on motility (either accelerated or impaired migration) and metabolism (measured by Alamar Blue). Focusing in greater detail on the 101 genes that accelerate migration extensive validation assays were performed reducing the set of high confidence genes to 31. Signaling network analysis of these genes reveals the Beta-catenin pathway is heavily involved. Analysis of published breast tumour microarray data has directed our immediate priorities towards a number of genes that were hits in our screen and identified in the arrays. The signaling pathways regulating cell migration of these new targets remains to be elucidated.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2007
Accession Number
ADA469757

Entities

People

  • Kaylene J. Simpson

Organizations

  • Harvard Medical School

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Cytoskeleton
  • Diseases And Disorders
  • Epithelial Cells
  • Families (Human)
  • Health Services
  • Mammary Glands
  • Metabolism
  • Neoplasms
  • Throughput
  • Validation
  • Wound Healing

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and genetic basis of cancer.