Metabolic Mapping of Breast Cancer with Multiphoton Spectral and Lifetime Imaging

Abstract

Currently we designed and implemented a combined spectral and lifetime imaging system which combines the benefits of multiphoton microscopy, spectral discrimination, lifetime analysis and temporal information. This system has efficiencies of > 50% and capability to collect broad spectrums (from 380nm-700nm). We determined the fluorescence source under our imaging condition is from NADH by this combined spectral and lifetime imaging system. Furthermore, we validated the accuracy of our lifetime analysis method. Our preliminary results of metabolic mapping in different human breast cell lines via fluorescence lifetime measurement of Co-Enzyme NADH showed very interesting results in differences of the lifetime and contribution of bound NADH between human normal breast cell line (MCF10a) and human breast cancer cell lines (T47D and MBA_MD_231). A 2-Deoxy-D-glucose (2DG) perturbation study is underway to link these finds with glycolysis.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2007
Accession Number
ADA469761

Entities

People

  • Long Yan

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Confocal Microscopy
  • Detectors
  • Health Services
  • Measurement
  • Microscopy
  • Neoplasms
  • Optics
  • Three Dimensional

Readers

  • Medical Imaging.
  • Molecular Biology and Genetics
  • Molecular and Cellular Biology