In Vivo Role of Six1 in Mammary Gland Tumorigenesis
Abstract
Homeobox transcription factor Six 1 has been identified as a critical mediator of tumorigenesis and metastasis in a number of organ systems and has been implicated in epithelial to mesenchymal transitions (EMT) during normal development. Our research is aimed at utilizing mouse models to understand its role in the onset and progression of breast cancer. Most significantly, we have determined that Six 1 is indeed sufficient to induce tumor formation in the mammary glands of mice genetically engineered to inducibly overexpress the gene. The latency for tumor formation is between 12-15 months and the tumors that arise in these animals are very aggressive and have morphological features of EMT, a phenomenon that has recently been suggested to contribute to metastasis. Further molecular analysis of these tumors will allow us to more carefully dissect the role of Six 1 in tumor onset. Additionally, in a number of animals that do not develop tumors, a hyperplastic phenotype is observed. Future experiments will involve turning off Six 1 expression after tumor formation to determine if Six 1 is required for tumor maintenance, thus determining the potential benefits of targeting Six 1 in a therapeutic setting.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2007
- Accession Number
- ADA469764
Entities
People
- Erica Mccoy
Organizations
- University of Colorado Health