Sonic Hedgehog Signaling Promotes Tumor Growth
Abstract
We have shown that the Sonic hedgehog (Shh) signaling pathway is absolutely required for normal prostate development and our studies performed with the support of the DOD New Investigator award indicate that Shh signaling promotes tumor growth. This proposal addresses the hypothesis that Sonic hedgehog signaling promotes tumor growth by activating stromal cell gene expression. To address this hypothesis, we have developed the DLPlacZ-LNCaP xenograft, a model that provides us with the opportunity to selectively assay gene expression in the stromal and epithelial compartments of the tumor using species specific PCR primers and to make specific modifications in stromal cell gene expression. We will use this model to: (1) determine whether Shh promotes tumor growth by activating expression of Gli-1 in the tumor stromal cells; (2) characterize the mechanisms by which tumor growth is promoted; and (3) examine the action of a specific stromal Shh target genes in tumor growth.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2007
- Accession Number
- ADA469894
Entities
People
- Wade Bushman
Organizations
- University of Wisconsin–Madison