Biological Function of Plasma Kallikrein in Mammary Gland Stromal Development and Tumor Metastasis

Abstract

The plasminogen cascade of serine proteases has been affiliated in the mammary gland with both development and tumorigenesis. We have found that the dominant plasminogen activator during mammary gland stromal involution is plasma kallikrein (PKal), and that active PKal appears in connective tissue-type mast cells in the mammary stroma during different phases of development. Examination of the extra hepatic expression of PKal has shown that PKal message is present in the mammary gland, and that increased expression levels correlate to periods of stromal remodeling. Additionally, an inhibitor of PKal that has been demonstrated to diminish mammary gland involution may be used to characterize PKal expression in the mammary gland as well as to identify targets of PKal activity during involution. Furthermore, mast cells are required for normal mammary duct branching morphogenesis during puberty. Lastly, a PKal knockout mouse has been produced that has the unexpected consequence of embryonic lethality.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2007
Accession Number
ADA469962

Entities

People

  • Jennifer Lilla

Organizations

  • University of California, San Francisco

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blood
  • Blood Coagulation Factors
  • Breast Cancer
  • Cells
  • Cellular Structures
  • Connective Tissue
  • Embryos
  • Inhibitors
  • Lethality
  • Mammary Glands
  • Mast Cells
  • Neoplasms
  • Plasminogen
  • Polymerase Chain Reaction
  • Tissues

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and Cellular Biochemistry