CTL-Tumor Cell Interaction: The Generation of Molecular Probes Capable of Monitoring the HLA-A*0201-HER-2/neu Peptide Complex
Abstract
The overall goal of this project is to probe the CTL - tumor cell interaction by generating scFv probes that are able to recognize the HLA-A*0201-HER-2/neu369-377 peptide complex. In the second 12-month funding period (Feb 2005 - Feb 2006) scFv 2.3.5-58-53 was isolated following site-directed mutagenesis in the VL CDR3 and VL CDR1 of HLA-A*0201 HER 2/neu369-377 complex-specific scFv 2.3.5. In the 12-month period covered by this report (Feb 2006 - Feb 2007), I aimed to further improve the recognition of HLA-A*0201-HER-2/neu369-377 complexes by scFv 2.3.5-58-53. To this end an scFv-Fc construct was utilized in an attempt to improve the avidity of the scFv for HLA-A*0201 :HER-2/neu369-377 complexes expressed on tumor cells. Although scFv 2.3.5-58-53-Fc demonstrated modestly enhanced reactivity with HER-2/neu369-377 peptide-pulsed T2 cells when compared with scFv 2.3.5-58-53 it did not exhibit enhanced reactivity with HLA-A2+HER-2/neu+ tumor cells PCA-30 and MDA-MB-231. We are actively investigating several possibilities that may account for this discrepancy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2007
- Accession Number
- ADA470245
Entities
People
- Eric C. Ko
Organizations
- Roswell Park Comprehensive Cancer Center