The Role of ADAM9 in Tumor-Stromal Interactions in Breast Cancer

Abstract

The tasks outlined in the Statement of Work for the first year of research on the action of ADAM9 isoforms in tumor-stromal interactions encompassed the creation of reagents to properly and thoroughly study the problem as well as preliminary work studying exogenous ADAM9 in a cell-based system. The major developments during this research period were preliminary data showing that the longer form of ADAM9 (ADAM9-L) enhances the migration of breast cancer cells and that this is dependent on it's metalloprotease activity and the development of an ADAM9-S(secreted form) antibody which allows us to detect both isoforms endogenously allowing for the development of more rigorous models using siRNA and endogenous protein to more thoroughly test our hypothesis.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2007
Accession Number
ADA470594

Entities

People

  • Jessica L. Fry

Organizations

  • Beth Israel Deaconess Medical Center

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Movement
  • Cells
  • Culture Media
  • Diseases And Disorders
  • Drug Therapy
  • Immunohistochemistry
  • Migration
  • Neoplasms
  • Proteins
  • Stromal Cells
  • Tissues

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics
  • Technical Research and Report Writing.