Magnetic Resonance Spectroscopy (MRS) of Prostatic Fluids for Early Detection of Prostate Cancer

Abstract

In this study, the metabolic profiles of expressed prostatic secretions (EPS) from 52 men with prostate cancer (PCa) and from 26 healthy controls were analyzed using quantitative proton nuclear magnetic resonance spectroscopy (1H-NMRS). The metabolites quantified included citrate, spermine, myo-inositol, lactate, alanine, phosphocholine, glutamine, acetate, and hydroxybutyrate. Logistic regression (LR) was used to model the risk of PCa based on metabolite concentrations while adjusting for age. The LR models indicated that the absolute concentrations of citrate, myo-inositol, and spermine were highly predictive of PCa and inversely related to the risk of PCa. The areas under the receiver operating characteristic curves (AUROC) for citrate, myo-inositol and spermine were 0.89, 0.87, and 0.79, respectively. At 90% sensitivity, these metabolites had specificities of 74%, 51% and 34%, respectively. The LR analysis indicated that absolute levels of these three metabolites were independent of age. The results indicate that citrate, myo-inositol and spermine are potentially important markers of PCa in human EPS. Further, the absolute concentration of these metabolites in EPS appears to be independent of age, increasing the potential utility of these markers due to elimination of age as a confounding variable.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2007
Accession Number
ADA470954

Entities

People

  • Colin O'donnell
  • Douglas J. Kominsky
  • E. D. Crawford
  • Eduard J. Gamito
  • Holly Sullivan
  • Jaimi L. Brown
  • Natalie J. Serkova
  • Richard H. Jones
  • Spencer Green
  • Tammy Hedlund

Organizations

  • University of Colorado Health

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Body Fluids
  • Chemical Shifts
  • Diseases And Disorders
  • Glutamine
  • High Resolution
  • Magnetic Resonance
  • Metabolites
  • Neoplasms
  • Nuclear Magnetic Resonance
  • Prostate
  • Prostate Cancer
  • Resonance
  • Secretion
  • Sensitivity
  • Spectroscopy

Readers

  • Analytical Chemistry
  • Prostate Cancer Biology.
  • Regression Analysis.