The Role of the POZ-ZF Transcription Factor Kaiso in Breast Cell Proliferation and Tumorigenesis
Abstract
Kaiso is a novel member of the POZ-ZF family of transcription factors, first identified as a binding partner to the p120ctn cell adhesion co-factor. Preliminary work in our laboratory indicated that Kaiso was misexpressed in ~40% of human breast tumors and identified cyclin D1 as a putative Kaiso target gene. The objective of this project is to determine the mechanism by which Kaiso regulates cyclin D1 expression and how this affects breast cell proliferation and tumorigenesis. Using techniques such as artificial promoter assays, electrophoretic mobility shift assays and semi-quantitative PCR we sought to determine how Kaiso regulates cyclin D1 promoter binding and expression. Our work to date demonstrates that Kaiso transcriptionally represses the cyclin D1 promoter through it's bimodal binding properties (binding to both sequence specific sites and methylated CpG sites within the promoter). We have also demonstrated that Kaiso and p120ctn activity may be modulating canonical Wnt signaling and activation of cyclin D1.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2007
- Accession Number
- ADA471106
Entities
People
- Michelle I. Anstey
Organizations
- McMaster University