Entrapment of Enzymes and Carbon Nanotubes in Biologically Synthesized Silica: Glucose Oxidase-catalyzed Direct Electron Transfer, Preprint
Abstract
This work demonstrates a new approach for building bio-inorganic interfaces by integrating biomimetically-derived silica with single-walled carbon nanotubes to create a conductive matrix for immobilization of enzymes. Such a strategy not only allows simple integration into bio-devices but presents an opportunity to intimately interface an enzyme and manifest direct electron transfer features. Biologically-templated silica/carbon nanotube/enzyme composites were evaluated electrochemically and characterized by means of X-ray photoelectron spectroscopy. Voltammetry of the composites displayed stable oxidation and reduction peaks at an optimal potential close to that of the FAC/FADS(sub 2) cofactor of immobilized glucose oxidase. The immobilization stabilized enzyme activity for a period of one month and retained catalytic activity towards the oxidation of glucose. It was demonstrated that the resulting composite can be successfully integrated into functional bio-electrodes for biosensor and biofuel cell applications.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2007
- Accession Number
- ADA471255
Entities
People
- Dmitri Invitski
- Glenn R. Johnson
- Heather R. Luckarift
- Kateryna Artyuskova
- Plamen Atanassov
- Rosalba A. Rincon
Organizations
- University of New Mexico