The Regulation of JAB1 and Its Role in Breast Cancer
Abstract
The purpose of the research done has been to determine the mechanism for overexpression of JAB1 through transcription factor analysis and FISH as well as the role of JAB1 in resistance. The major findings thus far are 1) The promoter region of JAB1 was analyzed and key transcription factors were identified that may drive JAB1 expression, CEBP alpha and GATA1 2) We have performed FISH on a number of breast cancer cell lines and patient fine Needle aspirations and have seen amplification of the JAB1 locus 3) JAB1 overexpression confers resistance to Herceptin in breast cancer cell lines SKBR3 and BT474, and inhibition of JAB1 increased the efficacy of Herceptin mediated G1 arrest and p27 accumulation. We completed the tasks listed for this time period and are on track as indicated in the SOW.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2007
- Accession Number
- ADA471501
Entities
People
- Terry J. Johnson
Organizations
- The University of Texas MD Anderson Cancer Center