The Role of Protein Elongation Factor eEF1A2 in Breast Cancer
Abstract
The overall goal of the project is to explore the role of protein elongation factor eEF1A2 in breast tumor development and to determine whether eEF1A2 is a useful breast cancer prognostic factor. eEF1A2 is one of two members of the eEF1A family of proteins (eEF1A1 and eEF1A2) that bind amino-acylated tRNA and facilitate their recruitment to the ribosome during protein translation elongation. We have identified eEF1A2 as a novel breast cancer oncogene. Supporting this idea are our observations that: a) eEF1A2 expression is increased in approximately 50% of human breast tumors; b) eEF1A2 protein expression is a prognostic marker for breast cancer survival; c) eEF1A2 enhances the growth rate of malignant breast cells; d) eEF1A2 inhibits anoikis; e) eEF1A1 activates the Akt/PKB serine/threonine kinase; e) siRNA that inactivate eEF1A2 and inhibit the in vitro growth of breast cell lines; and f) eEF1A2 expression causes rearrangement of the actin cytoskeleton. Taken together, our observations indicate that eEF1A2 is likely to play a causal role in the development of breast cancer and that it is a likely target for breast cancer therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2006
- Accession Number
- ADA471503
Entities
People
- Jonathan M. Lee