Characterization of Novel Genes Within 8P11-12 Amplicon in Breast Cancer
Abstract
The development of breast cancer is associated with gene amplification and overexpression that are %believe to have a causative role in oncogenesis. An important challenge in breast cancer research is to identify and characterize these genetic changes. Focal amplifications involving chromosome 8p11-p12 occur in approximately 15tilde20% of primary uncultured human breast cancers. Recently we have undertaken a detailed genomic and expression analysis of the 8p11-p12 amplicon in breast cancer cell lines and identified several novel candidate genes including TC-1 and FLJ14299. We observed that TC-1 is located at the common core-amplified domain of the 8p11-12 region and overexpressed in the subset of breast cancer cells. Furthermore we have found that TC-1 has properties of an oncogene: TC-1 expression in normal mammary epithelial cell line MCF10A increases growth rate and allows growth in soft agar. Notably suppression of TC-1 expression by siRNA inhibited cell proliferation in TC-1 over expressing breast cancer cell lines. Our recent date also suggested that TC-I over-expression is associated with the enhanced expression of a subset of beta-catenin target genes in breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2007
- Accession Number
- ADA471532
Entities
People
- Zeng-quan Yang
Organizations
- Wayne State University