The Role of Src in Mammary Epithelial Tumorigenesis

Abstract

The levels and activity of Src non-receptor tyrosine kinases are frequently found to be aberrantly elevated in mammary carcinoma cells compared to non-tumorigenic mammary epithelial cells. However, the role that Src family kinases play in tumor progression remains unclear. The overall objective of this study is to better elucidate the role that endogenous c-Src plays in mammary epithelial tumorigenesis. Our lab has found that genetic and pharmacological inhibition of Src in tumor cells that mimic the early stages of tumorigenesis (T4-2 cells), when cultured in a protein rich extracellular matrix (3D-rBM; Matrigel(TradeMark)), formed organized, polar, multicellular spheroid structures (termed 'acini') similar to the physiological lobular-aveoli structures found in the mammary tissue. Additionally, more invasive carcinoma cells (MDA-MB-231 cells) whereby Src signaling was pharmacologically or genetically inhibited were unable to form actin-rich invasive structures in 3D-rBM culture.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2007
Accession Number
ADA471564

Entities

People

  • Leonard Kusdra

Organizations

  • University of California, Berkeley

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Colon Cancer
  • Culture Techniques
  • Enzyme Inhibitors
  • Epithelial Cells
  • Fibroblasts
  • Inhibition
  • Membranes
  • Neoplasms
  • Proteins
  • Three Dimensional

Fields of Study

  • Biology
  • Chemistry

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology