EPR Assembly of Microgel for FRET Imaging of Breast Cancer

Abstract

The purpose of this project is to develop a new process for identifying breast cancer. This process should be at least as convenient as mammography for the patient more sensitive for detection of the smallest and earliest tumors, and more accurate in distinguishing tumors from normal tissues. First the tumor site is marked by entrapment of a polymer-PNA conjugate due to EPR effect. Based on the longer retention of the polymer conjugate in tumors versus normal tissue, a second polymer-PNA conjugate containing the complementary PNA sequence is administered. This second conjugate will interact with first conjugate due to complementary base pairing to form insoluble and intermolecular polymer networks (microgel). Theses microgels can then be used as targets to deliver another reagents for imaging. In first tow years of grants, wer have completed the synthesis of copolymer, polymer conjugates labeled with hilyte fluorescein dyes and polymer-PNA conjugates. We have also developed a non-inevasive method of investigating EPR by skinscan. We have initiated studies with polymer-PNA conjugates.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2007
Accession Number
ADA471769

Entities

People

  • Stanley Stein

Organizations

  • Rutgers University–New Brunswick

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Blood
  • Chemical Synthesis
  • Chemistry
  • Copolymers
  • Detection
  • Dielectric Polymers
  • Energy Transfer
  • Ethylene Glycol
  • Fluorescence
  • Fluorophores
  • Liquid Chromatography
  • Mammary Glands
  • Molecular Weight
  • Molecules
  • Polyethylene Glycols
  • Polymers
  • Transition Temperature

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Nanocomposite Materials Science
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Microelectronics