NF-1 Dependent Gene Regulation in Drosophila Melanogaster

Abstract

Neurofibromatosis type 1 (NF1) is characterized by benign but disfiguring skin tumors, pigmentation defects and learning disabilities, as well as increased risk of brain tumors. The NF1 tumor suppressor protein (neurofibromin) inhibits Ras, a protein that is overactive in a wide variety of human cancers. NF1 also controls levels of cyclic AMP, an important intracellular messenger involved in cell growth and learning. We have used an Affymetrix whole genome chip, containing all 13,500 genes of the fruit fly Drosophila, to identify 93 genes with altered expression patterns in flies that have no NF1 protein compared to normal wild type flies. Importantly, expression of half these genes is restored to normal levels when human NF1 is expressed in flies with no NF1. At least a quarter of these genes are involved in nervous system function or development.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2004
Accession Number
ADA471891

Entities

People

  • Yi Zhong

Organizations

  • Cold Spring Harbor Laboratory

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biological Sciences
  • Cells
  • Chemistry
  • Chromosomes
  • Diptera
  • Dna Microarrays
  • Drosophila
  • Genetics
  • Health Services
  • Mrna
  • Neoplasms
  • Nervous System
  • Neuromuscular Diseases
  • Peripheral Nervous System
  • Proteins
  • Transcription Factors

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology
  • Molecular and genetic basis of cancer.