Role of Stat5a in Differentiation of Human Breast Cancer
Abstract
Stat5 is closely involved in mammary gland differentiation and lactation. We have previously shown that active Stat5 is lost during breast cancer progression and this loss is associated with a more aggressive disease status. In this study, we propose to investigate the ability of active Stat5 to induce differentiation as a means to suppress invasion and metastasis of breast cancer cell lines. Using a constitutively active Stat5a that is tyrosine phosphorylated and transcriptionally active in the absence of prolactin stimulation, we hypothesize that over expression of active Stat5 will correlate with increased expression of differentiation markers and reduced invasion in vitro and in vivo. We have generated two constitutively active Stat5a constructs, Stat5a-S710F and Stat5a-3ser, and have determined that Sta5a-3ser has a greater potential to be active in the absence of prolactin stimulation. We have generated lentiviral, adenoviral, and MDA-MB-231 stable cell lines expressing these constructs and are in the process of initiating both in vitro and in vivo differentiation and invasion studies outlined in the original proposal.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2007
- Accession Number
- ADA472072
Entities
People
- Amy Ryder
Organizations
- Thomas Jefferson University