Signaling Crosstalk: A Live in Situ Analysis of the Temporal and Spatial Regulation of Key Pathways in Human Breast Cancer Progression
Abstract
Signal transduction networks such as the PI3K-AKT and EGFR pathways are important regulators of cell fate decisions, including cell proliferation, differentiation, apoptosis, and homeostasis. Furthermore, these pathways integrate and influence one another when cells are within an appropriate microenvironment. Using a proteomic approach, we identify stratifin, a protein which regulates AKT and EGFR signaling as well as the cell cycle, to be upregulated in T4-2 cells cultured in 3D lrECM. Expression of stratifin decreases to S1 levels upon phenotypic reversion; these effects were not seen when cells were cultured in 2D, supporting a possible role of stratifin in crosstalk. In the mouse mammary gland, stratifin expression was restricted to myoepithelial cells, and was expressed predominantly during periods of branching morphogenesis and ductal infiltration. Taken together, these data suggest a novel role of stratifin in epithelial cell proliferation and migration. The signaling networks regulated by stratifin will be assessed by shRNA knockdown in T4-2 cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2007
- Accession Number
- ADA472111
Entities
People
- Aaron Boudreau
Organizations
- University of California, Berkeley