Interfering with DNA Damage Signals: Radiosensitizing Prostate Cancer using Small Peptides
Abstract
Our focus of this project is to characterize a newly developed small peptide on its ability to function as a powerful radiosensitizer. Radio sensitivity is mainly controlled by a kinase named ATM and its phosphorylation of downstream targets, including Structural Maintenance of Chromosomal protein on (SMC1). Previously we have demonstrated that small fusion peptides containing SMC1 phosphorylation sequences can inhibit ATM activity. We have characterized the inhibitory effect of the THM-SMC1 peptide on cellular response to radiation and found the peptide can abolish radiation induced S- phase checkpoint and decrease prostate tumor cell clonogenic survival. During the last performance period, we further performed experiments focusing of the magnitude of peptide sensitization and the effect on the other cell cycle checkpoints. We have demonstrated the wild type SMC1 peptide linked with a tumor homing motif can significantly increase prostate tumor radiosensitivity. Future experiments will be focusing on mechanisms and the in vivo activity of the peptides.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2006
- Accession Number
- ADA472316
Entities
People
- Bo Xu
Organizations
- Louisiana State University