Characterization of Odin, a Novel Inhibitory Molecule, in EGF Receptor Signaling

Abstract

Protein phosphorylation plays a key role in the regulation of the function of the proteins and the control of wild range of cellular process. Odin, one of signaling molecules identified in EGF receptor signaling pathway, functions as a negative regulator of growth factor receptor signaling pathways. To dissect the molecular mechanism of Odin in signaling pathway and its biological function, we carried out in vitro kinase assay based on peptide array and identified two new tyrosine phosphorylation site of Odin by c-Src. We also developed two cell lines to study the protein complex of Odin by mass spectrometry. In the future, we seek to identify the domain of Grb2 binding to Odin and the interacting protein(s) of Odin and its phosphorylation site. We also want to characterize the role of the phosphorylation in controlling the function of Odin.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2006
Accession Number
ADA472333

Entities

People

  • Akhilesh Pandey
  • Jun Zhong

Organizations

  • Johns Hopkins University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anatomy
  • Antibodies
  • Biological Sciences
  • Biomedical Research
  • Cell Line
  • Cell Movement
  • Cells
  • Cells (Biology)
  • Chemical Compounds
  • Enzymes
  • Growth Factors
  • Kinases
  • Mass Spectrometry
  • Molecules
  • Phosphorylation
  • Spectrometry
  • Tyrosine

Fields of Study

  • Biology
  • Chemistry

Readers

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