Identification of Genes Regulating the Development of Breast Cancer
Abstract
Breast cancer develops through multiple steps which are rigorously controlled by genetic factors. It is essential to identify and characterize genes controlling the development of breast cancer to better understand factors affecting tumor susceptibility and contribute to better diagnosis and treatment. We are using a well characterized mouse model, ApcMin/+ mice, to identify genes important for breast cancer progression and development. As the first step to identify modifier gene, we backcrossed B6-ApcMin/+ to the resistant strain FVB. Four novel modifier loci have been mapped to influence different aspects of mammary tumor development in ApcMin/+ mice. Analysis of tumor development in a backcross of (FVBB6 ApcMin/+) x B6 ApcMin/+ mice has identified a modifier on chromosome 9 that significantly affects tumor multiplicity, and a modifier on chromosome 4 that significantly affects tumor latency and affects tumor number with suggestive significance. This modifier was also identified in a backcross involving 129X1/SvJ and B6 ApcMin/+ mice. A modifier on chromosome 18 specifically affects tumor latency but not tumor number. Kaplan-Meier analysis suggests there is at least an additive interaction affecting tumor latency between the loci on chromosomes 4 and 18. I also identified a modifier locus on chromosome 6 that interacts with the loci on chromosome 4 and chromosome 9 to affect tumor number. To further identify genes underlying these modifier loci, I genereated and congenic mice on chromosome 4 and 9. Preliminary analysis provide evidence for the modifier on chromosome 4.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2007
- Accession Number
- ADA472358
Entities
People
- Amy Moser
- Hua Wang
Organizations
- University of Wisconsin–Madison