Establishment of an 'In Vitro Cell-Based System' to Assay Radiation Sensitivity in Breast Cancer
Abstract
The purpose of this proposal is to develop an 'in vitro cell-based system' to study radio-resistance in sporadic breast cancer. By performing colony formation assays in response to X-ray treatment we have shown that there is a wide range of sensitivities to this form of treatment. Interestingly, we have also observed that tp53 status does not accurately predict response to radiation treatment. We have sensitized one of our most radio-resistant breast cancer cell lines to X-ray treatment by using RNAi-based technology to inhibit Artemis function. In the future, we feel these observations will have a significant clinical impact on the way radiation oncologists treat breast cancer patients.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2007
- Accession Number
- ADA472420
Entities
People
- Gregory T. Langland
Organizations
- University of California, Berkeley