Imaging Primary Prostate Cancer and Bone Metastasis
Abstract
The overall objective of the proposed research is to develop positron emitter labeled bombesin (BBN) analogs with high affinity for the GRP receptor GRPR for microPET imaging of both androgen dependent and androgen independent prostate cancer xenografted mice. Specific Aims: (1) Design, synthesize, and characterize positron emitting bombesin analogs, labeled with copper-64 or fluorine-18; (2) Conduct in vitro studies of copper-64 and fluorine-18 labeled bombesin analogs to evaluate the effect of modification and radiolabeling on the receptor binding affinity and specificity; (3) Evaluate in vivo efficacy of these novel radiopharmaceuticals in the murine PC-3 and CWR22 human prostate cancer xenograft models. Major Findings: In year 1, we labeled Lys3-BBN with 64Cu for imaging both subcutaneous PC-3 (GRPR+) and CWR22 (GRPR-) tumors. In year 2, we further tested a series of BBN analogs and fully characterized 64Cu-DOTA-Aca-BBN(7-14). In year 3, we labeled several BBN analogs with F-18 for prostate cancer imaging. By testing a series of BBN analogs, we identified at least one 18F- and one 64Cu-labeled bombesin peptide tracers that can specifically localize to GRPR expressing tumors. These new peptide tracers have the potential to be translated into clinical settings for lesion detection and quantification of GRPR level.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2007
- Accession Number
- ADA472718
Entities
People
- Xiaoyuan Chen
Organizations
- Stanford University