Identifying Molecular Targets for Chemoprevention in a Rat Model
Abstract
The purpose of this grant is to determine the molecular events that occur in the dorsal and ventral lobes of the rat prostate gland after 20 weeks of exposure to PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine). PhIP is a potent inducer of mutations in the rate prostate where we have previously shown that it forms bulky DNA adducts. The scope of this research includes: 1)Generation of a rat model, 2) Analysis of the rat prostate after 20 weeks of PhIP, and 3) Gene chip microarray analysis. To date, we have completed the histopathologic analysis of the PhIP induced prostate pathology, we have generated an inflammatory/Atrophyl proliferation model, and we have acquired data that refutes a previous model of neoplastic progression. These results have been published in the journal, Neoplasia. In addition, we have completed approximately 80% of the study to investigate the molecular changes occurring in the prostate during PhIP exposure. Animal exposures have been completed and we are awaiting completion of the microarray analysis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2007
- Accession Number
- ADA473649
Entities
People
- Ralph W. Devere White
Organizations
- University of California