Molecular Mechanism by Which Retinoids Prevent Breast Cancer Development
Abstract
We investigated whether rexinoids suppress the growth of breast cells and how they act to inhibit breast cancer development. First we measured the growth suppressive activity of rexinoids (RO 25J386 LGDIO69 and LGIOO268) on the growth of breast cells (normal and malignant). Second we identified the genes induced by rexinoids that could be related to the cell growth inhibition. By MTS assay we found that all three compounds (Ro25-7386, LGDIO69and LG100268) inhibited significantly the growth of normal breast cells HMEC, at 10 uM suggesting the chemopreventive property of the rexinoids. The growth of MCF-7 cells was inhibited by Ro25-7386. T47D breast cancer cells were found to be growth suppressive by Ro25-7386 and LGDIO69; they strongly suppressed the cell growth by dose-dependent manner. Surpnsingly LGDIO69 also induced a mild inhibition (20 % growth inhibition) of MDA-MB-231 at 10 uM suggesting that LGDIO69 can inhibit the ER-negative breast cancer cell growth in vitro. Later we performed the Affymetrix microarray to identify the genes induced by rexinoids. We found several interesting genes induced by the rexinoids.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2007
- Accession Number
- ADA473735
Entities
People
- Hye-sook
Organizations
- University of Texas at Austin