Forensic Discrimination of 25 Isolates of Burkholderia mallei

Abstract

In this study, the subspecies differentiation of 25 isolates of Burkholderia mallei was attempted based on their ribotype polymorphisms. The isolates were from human and equine infections that occurred at various times around the world. DNA samples from each isolate were digested with PstI and EcoRI enzymes and probed with an Escherichia coli-derived 18-mer rDNA sequence to identify diagnostic fragments. Seventeen distinct ribotypes were identified from the combined data obtained with the two restriction enzymes. The results demonstrate the general utility of ribotyping for the subspecies identification of B. mallei isolates.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2007
Accession Number
ADA473746

Entities

People

  • Jennifer M. Minter
  • Steven P. Harvey

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Bacteria
  • Discrimination
  • Diseases And Disorders
  • Epidemiology
  • Escherichia Coli
  • Geography
  • Health Services
  • Identification
  • Infection
  • Microbiology
  • Microorganisms
  • New England
  • Public Health
  • Sequences
  • Standards
  • United States
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular Genetics