The Functions of BRCA2 in Homologous Recombinational Repair

Abstract

During this no cost extend funding period we concluded that BRCA2-Rad51 interaction is crucial for HR repair and multiple regions of BRCA2 protein are involved in regulating HR repair. A manuscript has been prepared for submission. In addition We found that upon replication stresses DNA-PKcs is phosphorylated and phosphorylated DNA-PKcs co-localizes with Brca1. The possible interaction between DNA-PKcs and Brca1 was confirmed in the co- immunoprecipitation (co-IP) analysis showing that DNA-PKcs could be co-precipitated with the alpha-Brca1 antibody. Furthermore GST-Brca1 fusion proteins covering different region of Brca1 were mixed with HeLa nuclear extract followed by co-IP with alpha-DNA-PKcs antibody and western against alpha-GST antibody. The result showed that Brca1 fragment #5 may potentially interact directly with DNA-PKcs. We plan to explore this direction in the future.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2007
Accession Number
ADA473770

Entities

People

  • David J. Chen

Organizations

  • University of Texas at Dallas

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cells
  • Chemical Compounds
  • Coverings
  • Department Of Defense
  • Electronic Mail
  • Frequency
  • Information Operations
  • Macromolecules
  • Molecular Weight
  • Molecules
  • Multiprotein Complexes
  • Protein-Protein Interactions
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics