Eosinophil Granular Protein(s) Modulate Tumor Metastasis Marker Gene Expression
Abstract
Multicellular tumor spheroids are a suitable tumor model system for investigating the effect of anti-cancer agents on tumor growth. The overall objective of the present investigation is to examine the effect of isolated eosinophil toxic granular protein(s) and cytokines IL-4, IL-10, IL-12, TNF+/- on markers of tumor growth and metastasis (e.g. erbB2, cyclin D1, cyclin E). Early studies concentrated on the development of the spheroid model. Optimization of spheroid size, shape and number was emphasized in year 1. Of the two non-metastatic breast cell lines selected, the MCF-7 tumor line formed ideal spherical tumors, while the T-47D cells took longer to form round spheroids which were half the size of the MCF-7 MTS. The metastatic cell line MDA-MB-468 fared to grow. The data are inconclusive regarding proliferative state of the MTS fractions. They were too few cells in the early fractions. All eight eosinophil cell lines were examined and ready for batch culture for protein isolation. Additional experiments are needed for optimal standardization of the MTS models.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2007
- Accession Number
- ADA473779
Entities
People
- Paulette Furbert-harris
Organizations
- Howard University